Application

This Anti-Parvalbumin Antibody, clone 4E9.2 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Parvalbumin.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Parvalbumin in human kidney, rat kidney, and mouse cerebral cortex tissue sections.

General description

Parvalbumin alpha (UniProt P02625; also known as PV) is encoded by the Pvalb (also known as Pva) gene (Gene ID 25269) in rat species. Parvalbumin alpha is a small (a.a. 2-110; Met1 removed posttranslationally) calcium-binding protein. Parvalbumin alpha is composed almost entirely of two EF-hand domains (a.a. 39-74 & 78-110) and is structurally related to calmodulin and troponin C. Parvalbumin (PV) is present in GABAergic interneurons in the nervous system, especially the reticular thalamus. PV interneurons are a subset of inhibitory GABAergic neurons that control the excitability of post-synaptic pyramidal neurons. Dysfunction or loss of PV interneurons is implicated in cognitive deficits associated with a number of well known psychiatric disorders, including schizophrenia, Alzheimer's disease, and depression.

Immunogen

KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of rat parvalbumin.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Purified

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected Parvalbumin in 10 µg of mouse brain tissue lysate.

Specificity

Clone 4E9.2 detects an epitope conserved among human, mouse, and rat species.

Target description

~12 kDa observed. 11.93 kDa (human), 11.80 kDa (mouse), 11.79 kDa (rat) calculated (Met1 removed). Uncharacterized band(s) may appear in some lysates.